As part of our Immune Health Program at the Allen Institute for Immunology, we generated RNA-seq data from > 1.8 million single cells obtained from 108 Pediatric, Young Adult, and Older Adult donors to generate a cross-age Cell Type atlas of Peripheral Blood Mononuclear Cells (PBMCs). The samples used in this study were obtained in collaboration with the Benaroya Research Institute and the University of Pennsylvania.
See the sections linked to the left for more details about our cohorts, data generation, cell type definitions, downloads, and how to cite this work.
We also provide instructions for utilizing CellTypist models generated from this atlas to label your own Human PBMC data.
To interactively explore our PBMC Cell Type Atlas, we provide the following visualization tools:
PBMC Cell Type Gene Expression
Quickly look up gene expression in our PBMC Cell type atlas with our Gene Expression Summary visualization tool.
Interactive UMAP Visualization
Visualize a UMAP projection of our dataset to inspect cell type labels, sample metadata, and gene expression patterns.
Differential expression viewer
Examine cell type-specific differences in gene expression due to subject age group, CMV infection status, or biological sex.
Clinical labs and metadata viewer
Investigate sample metadata and clinical lab results, including Clinical Blood Counts, a Metabolic Panel, and Lipid Panels generated for all subjects.